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VOCABULARY(Definitions are below)

biochemical  
     pathway
photosynthesis
reactants
products
byproducts
cellular
    respiration
energy forms
electromagnetic
   spectrum
wavelength
autotroph
heterotroph
chemiosmosis
citric acid
carbon fixation
Calvin Cycle
lumen
Factors
   affecting
   psyn. rate
glycolysis
chlorophyll
carotene
xanthophylls
chloroplast
thylakoid
granum
stroma
stoma
aerobic
anaerobic
fermentation
palisade and
  spongy   
       mesophyll
photolysis
psyn. formula
resp. formula
pyruvic acid
cristae
mitochondrion
matrix
Krebs cycle
ATP Synthase
Lactic acid
Ethyl alcohol
NADPH
ATP / ADP + Pi
NADH
LDR
LIR
ETC
CO2
O2
H+
e-
λ


energy . . . .
  dancing baby


 Jeopardy Game for Cell Energetics 

 Yeast Lab (this is the lab handed out in class...directions and questions)

Yeast Lab Data:                        (see below for Create-A-Graph data entry set-up)

BLOCK 1                    
CO2 Production 3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min
of Yeast Cultures                    
Dextrose Team 1 1 1 6 8 10 10.5 11 12 13 13
Dextrose Team 2 1 2 4 7 13 17 17 17 17 17
Dextrose Team 3  1 2 2 5 9 11 12 13 13.5 14
Dextrose Team 4  0.5 2 6 10 11 12 13 13.5 14 14
Dextrose Team 5 1 8 11 14 15 15.5 15.5 15.5    
Dextrose Team 6 1 2 3 5 8 10 11 12 13.5 14
Dextrose Team 7 1 1.5 3.5 6.5 8.5 10.5 11.5 12 13.5 14
Dextrose Team 8 1 2 3 4 4.5 5 5.5 6 7.5 8.5
Dextrose Average 0.9 2.6 4.8 7.4 9.9 11.4 12.1 12.6 13.1 13.5
                       
Lactose Team 1 1 1 1 1 1 1 1 1 1 1
Lactose Team 2 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
Lactose Team 3 1 1 1 1 1 1 1 1 1 1
Lactose Team 4 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Lactose Team 5 0.5 1 1 1 1 1 1 1 1 1
Lactose Team 6 1 1 1 1 1 1 1 1 1 1
Lactose Team 7 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
Lactose Team 8 1 1 1 1 1 1 1 1 1 1
Lactose Average 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7
                       
Maltose Team 1 1 1 4 6 7 8 9 10 11 12
Maltose Team 2 1 1 1 3 4 7 9 11 13 15
Maltose Team 3 1 1.5 2.5 3.5 5 7.5 9 10 10 12
Maltose Team 4 1 3 4 4.5 6 6.5 7 7 7.5 8
Maltose Team 5 1 3 6 7 8 8.5 8.5 8.5 8.5 8.5
Maltose Team 6 1 1.5 3 3.5 7 7 7.5 7.5 8 8
Maltose Team 7 0.2 0.3 0.5 1 1.5 2 2.5 3 4 4.5
Maltose Team 8 1.5 2 2.5 3 3.5 4 4 5.5 6 6
Maltose Average 1.0 1.7 2.9 3.9 5.3 6.3 7.1 7.8 8.5 9.3
                       
Sucrose Team 1 1 1 4 7 8 9 9.5 10 11 11
Sucrose Team 2 1 2 4 6 13 14 17 17 17 17
Sucrose Team 3 1.5 2.5 3.5 8 13 13.5 14.5 15 15 15
Sucrose Team 4 1 3.5 6 8 11 11 11.5 12 12.5 13
Sucrose Team 5 2 10 14 16 17 17 17 17 17 17
Sucrose Team 6 1.5 3.5 7 10 13 13.5 14 14.5 15 15
Sucrose Team 7 0.7 1.5 3 5.5 7.5 9.5 10.5 11 12 12.5
Sucrose Team 8 1.5 2 3 3.5 4 4.5 5 5 6 7.5
Sucrose Average 1.3 3.3 5.6 8.0 10.8 11.5 12.4 12.7 13.2 13.5
    3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min
                       
                       
BLOCK 3                    
CO2 Production 3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min
of Yeast Cultures                    
Dextrose Team 1 0.5 2 2 7 6 6 8 10.5 11 13
Dextrose Team 2 2 5.5 8 10 12 13        
Dextrose Team 3  1 1.5 3.5 5.5 7 8.5 10 10.5 11 11.5
Dextrose Team 4  0.3 1.5 4 6.5 9 10.5 11 12.5    
Dextrose Team 5 6.5 8.5 10.5 10.5 12 9.5 9      
Dextrose Team 6 1 4 8 12 16          
Dextrose Average 1.9 3.8 6.0 8.6 10.3 9.5 9.5 11.2 11.0 12.3
                       
Lactose Team 1 0.25 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Lactose Team 2 0 0 0 0 0 0 0 0 0 0
Lactose Team 3 1 1 1 1 1 1 1 1 1 1
Lactose Team 4 0.5 1 1 1.5 0.5 0.8 0.5 0.5    
Lactose Team 5 0 0 0 0 0 0 0 0 0  
Lactose Team 6 0 0 0 0 0 0        
Lactose Average 0.3 0.4 0.4 0.5 0.3 0.4 0.4 0.4 0.4 0.5
                       
Maltose Team 1 1 1 2 2.5 3 3 3.5 7 5 6
Maltose Team 2 1 2.5 3.5 5.5 7.5 8        
Maltose Team 3 1 1 2.5 5 5.5 6.5 7 7 8 8
Maltose Team 4 2 2.5 3 4 4.5 5.5 5.5 6    
Maltose Team 5 0 0 0 0 0 0 0 0    
Maltose Team 6 0.5 1 3 5 8 10 12 14 16  
Maltose Average 0.9 1.3 2.3 3.7 4.8 5.5 5.6 6.8 9.7 7.0
                       
Sucrose Team 1 1.5 3 5 5.5 6.5 7.5 9 10.5 12.5 13
Sucrose Team 2 1.5 2 5.5 6 6 7        
Sucrose Team 3 1 1.5 4 6.5 8 9.5 10.5 11 12 12.5
Sucrose Team 4 1 2.5 3.5 7 8 10 10.5 11    
Sucrose Team 5 6 10 12 12.5 12.2 10.5 10      
Sucrose Team 6 0.5 2.5 6 9 12 15        
Sucrose Average 1.9 3.6 6.0 7.8 8.8 9.9 10.0 10.8 12.3 12.8
    3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min
                       
                       
BLOCK 4                    
CO2 Production 3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min
of Yeast Cultures                    
Dextrose Team 1 1 2 3 4 4 5 5 6 7 10
Dextrose Team 2 7 9 11 12 12 14 15 17 17 17
Dextrose Team 3  1 7 9 11 11.5 12.5 13 13.5 14 15
Dextrose Team 4  1 2 3 4 6 8 9 9 9 10
Dextrose Team 5 0.5 4 5 10 10 11 12 12.5 13 14
Dextrose Team 6 1 2 5 9 9.5 11 11.5 12 12.5 13
Dextrose Average 1.9 4.3 6.0 8.3 8.8 10.3 10.9 11.7 12.1 13.2
                       
Lactose Team 1 1 2 2 2 2 2 2 2.5 2.5 3
Lactose Team 2 0 0 0 0 0 0 0 0 0 0
Lactose Team 3 0.1 0.1 0.1 0.1 0.1 0.1 0.2 0.2 0.1 0.2
Lactose Team 4 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
Lactose Team 5 0.1 0.1 0.2 0.3 0.3 0.3 0.3 0.3 0.3 0.3
Lactose Team 6 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Lactose Average 0.3 0.5 0.5 0.5 0.5 0.5 0.5 0.6 0.6 0.7
                       
Maltose Team 1 2 4 4 4 6 6 7 7.5 8.5 9
Maltose Team 2 5 6 9 10 9 10 12 13 15 17
Maltose Team 3 1.5 2 4 6 8.5 9.5 10.5 11 12.5 13
Maltose Team 4 0.5 1 3 3 5 5 6 7 7 8
Maltose Team 5 1 1 3 3 6 7 8 8 9 10
Maltose Team 6 1 3 4 5 7 8 8.5 9 9.5 10
Maltose Average 1.8 2.8 4.5 5.2 6.9 7.6 8.7 9.3 10.3 11.2
                       
Sucrose Team 1 1 4 9 10 11 11 11 12 12.5 13
Sucrose Team 2 6 10 10 11 11 12 14 14 16 16
Sucrose Team 3 2.5 8 10 11.5 12 13 13.5 14 14.5 15
Sucrose Team 4 0.5 2 3 5 7 9 9 10 11 12
Sucrose Team 5 1 3 4 6 9 9.5 10 10.5 11 12
Sucrose Team 6 0.5 5 10 10 11 12 12 12 12.5 12.5
Sucrose Average 1.9 5.3 7.7 8.9 10.2 11.1 11.6 12.1 12.9 13.4
    3 min 6 min  9 min 12 min 15 min 18 min 21 min 24 min 27 min 30 min

 How you need to set up your data in Create-A-Graph:
 
You may email this directly to me if you wish, or download it to yourself and attach it to an email to me.
 Honors: need two graphs: a
line graph of your group and of the class averages
CP:
Class avg. line graph only. 
 Finish other questions on lab and turn in.

 

Textbook reading (Honors):
               54 [ATP]
     113-118 [Light reactions]
     120-124 [Calvin cycle]
     130-136 [Glycolysis/Fermentation]
     137-144 [Aerobic respiration]

ATP ppt.  Know how this molecule delivers power.
 

Psyn/Resp ppt. (from class)
Print these slides (or the text from the "outline" tab) to study for your test on Nov. 3.
Illustrations, animations, and vocabulary on this page will supplement this.  Use your textbook for additional explanation.

Jeopardy ppt.

 



Synopsis of this unit:

The study of energy through living systems begins with the cell and how energy is obtained and used.  In this unit we study two major ideas:  photosynthesis and respiration.

    For phototrophs (a type of autotroph), light energy is the initial energy that's converted. 
   Light and H2O are used along with CO2 to produce a glucose molecule. 

   Heterotrophs cannot make their own food; they must eat their chemical energy (to get glucose).

Photosynthesis is how energy is first captured (from the sun) and converted to chemical energy by plants.  All other life benefits from this.  From this process, the chemical energy created is a sugar.
                                                         Light
                6
CO2 + 6H2O  à C6H12O6 + 6O2
    
                             reactant               reactant                    
product                  product

 
The above is a chemical equation, made up of different chemical formulas for each compound.
 
  It reads:
  "For every 6 carbon dioxides and 6 water molecules, you yield one glucose molecule and six
   diatomic oxygen molecules."

  In equations like the above, the compounds on the left of the arrow are called reactants,
  while the compounds on the right of the arrow are called products.

  Many times with the above photosynthesis equation, the term "light" is often put with the arrow.

 

Respiration is what happens to this sugar.  Whether you are a plant (that has just made its own sugar food) or another creature (that has just eaten your food), this sugar must now be broken down and converted ultimately into smaller power molecules that will drive much of the metabolic processes necessary for life to exist.  From glycolysis to aerobic respiration in the mitochondrion (or through the anaerobic process of fermentation), ATP is produced as the prized power molecule.

 C6H12O6 + 6O2 --> 6CO2 + 6H2O + 36ATP

Use the information below to help you with these concepts:



 


 A Bright Beginning; A Sweet Result
  How photosynthesis works


  Here's the classroom poster on p'syn.  It's just one power point slide.  You may download if you wish.  Zoom in on parts of the poster to view and read better.

  

 

This is excellent animation on the process of
photosynthesis.  Shows great detail of how the photosystems work in the thylakoids.

 

 

 

 THIS IS ATP:  Adenosine Triphosphate

Structure of ATP

 The image above is from
a great site explaining energy...

 

 
Here's your vocabulary.... handed to you with a silver spoon.  If you see a link by a definition, USE IT.

biochemical pathway-a series of chemical reactions that are related; the products of one reaction become the reactants of the next reaction.   (poster)  (cycle) (image)
reactants-elements or compounds that react together to produce a new product/compound. (explanation)
products-the results of reactants reacting together in a chemical process. (explanation)
byproducts-products produced by a reaction that are not needed.
aerobic- requiring oxygen (ex; aerobic respiration in mitochondrion)
anaerobic- not requiring oxygen (ex: glycolysis and fermentation)
cellular respiration-a process; cells get energy from carbs (sugars); O2 combines w/glucose to form water and CO2 while ATP is produced.
(image)
energy forms-chemical, light, sound, kinetic, thermal, electrical
electromagnetic spectrum-a scale of energy, measuring wavelength strength
(image)
wavelength- the distance from one peak to the next peak (or from trough to trough) of a wave of energy. (image)
                     [
λ- lambda; a Greek letter used to indicate "wavelength"]
autotroph-makes its own food
heterotroph- must eat (ingest) its own food
chemiosmosis- a process by which ADP + P is reassembled to ATP
carbon fixation-Converting atmospheric carbon (CO2) into an organic molecule
(like glucose) (image)
Factors affecting psyn. rate- light intensity and duration, temperature, amount of water, and amount of CO2

Leaf parts:
palisade mesophyll (or palisade parenchyma)- tightly packed vertical cells in top portion of leaf profile
(see image below)
spongy mesophyll (or spongy parenchyma) - loosely packed cells under palisades
stoma- opening in underside of leaves used for gas exchange
carotene- pigment used to capture light energy; orange-red in color.
xanthophyll-yellow pigment  (see more on pigments)


In the Chloroplast: (image below)  (details)
photosynthesis-process: light energy is used to produce chemical energy
                           in the form of a sugar; occurs in chloroplasts

chloroplast-organelle in which photosynthesis occurs
     >thylakoid-inner membrane of chloroplast where the LDR occurs
                   [
granum
- a stack of thylakoids (grana - pl.)]
                   [
chlorophyll- pigment found in chloroplast's thylakoid membranes
                      used to capture light energy; green in color
(image)]
                   [
lumen-fluid in thylakoid, where the buildup of H+ occurs. (image)]
                   LDR- light dependent reaction; occurs in thylakoid mem., needs
                   light to be activated, produces NADPH and ATP to drive the LIR.
 
                            (animation)

                   [photolysis- the splitting of water by light energy (in thylakoid)]
    >stroma- fluid surrounding thylakoids in chloroplast
    
     LIR- light independent reaction; in stroma fluid; also called Calvin cycle;
                   driven by NADPH and ATP from LIR to power cycle.  CO2 introduced, final product is glucose.
(image)

In the Cytoplasm:  (site)
glycolysis
-anaerobic process in the cytoplasm where glucose is split into
                2 pyruvic acids; a net gain of 2 ATP is produced, along with 2 NADH.
(image)
     pyruvic acid- the 3 C compound formed in glycolysis when glucose is split

In the Mitochondrion: (site)
mitochondrion
- organelle where aerobic respiration occurs.  Contains:
  
  cristae- inner membrane of mitochondrion where the ETC occurs
     matrix- inner fluid of mitochondrion, where the Krebs cycle occurs
                Krebs cycle- process in aerobic respiration; in matrix; produces 2-4 ATP (watch)
                        [
citric acid- a compound produced in the Krebs Cycle]

Also in the Cytoplasm:
fermentation- anaerobic process in the cytoplasm that allows glycolysis to run,
               2 ATP produced.
(image 1) (image 2) 
                       
Two types: Alcoholic and Lactic Acid:
    
Lactic acid- a byproduct of Lactic Acid Fermentation;
                         occurs in our muscle tissue.
(image)
     Ethyl alcohol- a byproduct of Alcoholic Fermentation;
                          occurs with yeast and bacteria.
(image)


 

Molecules to know:
                                                                
light
psyn. formula- 6CO2 + 6H2O  à C6H12O6 + 6O2


resp. formula- C6H12O6 + 6O2 à 6CO2 + 6H2O + 36ATP


ATP Synthase- an enzyme in the thylakoid membrane (of chloroplast) and cristae (of mitochondrion);
                           converts ADP + P to ATP

NADPH- the power carrying molecule that helps drive the Calvin cycle in photosynthesis.

ATP 1 ADP + Pi - Adenosine Triphosphate; the main energy carrying molecule
                            of respiration; can be used and reused  (reduced to Adenosine Diphosphate)
(image)

NADH - power molecule used in respiration (in mitochondrion) (watch)

ETC - Electron Transport Chain;  series of proteins and carriers in a membrane that transports electrons. 
               Used in thylakoids during LDR and in mitochondrion's cristae.

CO2
- 1) A source of carbon in photosynthesis,
          2) a byproduct of aerobic respiration,
          3)a byproduct of alcoholic fermentation (which is anaerobic)


O2 - oxygen;
            1) a byproduct of photolysis in the LDR,
            2) needed in aerobic respiration

H+  - a hydrogen ion (a proton); can be obtained from water by photolysis

   e-  -an electron; used to assemble molecules

 

 

Fermentation occurs (in cytosol) when NO O2 present
Sustains glycolysis: Keeps 2 ATPs  forming from each glucose
Two types:
Alcoholic ferm.
Some bacteria, yeast.  Byproducts: Ethyl alcohol and CO2
Lactic Acid ferm.
In your muscle tissue     Byproduct: Lactic acid



 

Contracting muscles must have ATP, and this comes from four sources:

    1. ATP stored in muscle--exhausted in a few seconds.
    2. ATP made from creatine phosphate--also minor, just lasts 10-15 seconds.
    3. ATP made from anaerobic breakdown of glycogen (a polymer of glucose).
    4. ATP made from aerobic breakdown of glycogen.

The last two sources of ATP have different characteristics. Anaerobic breakdown of glycogen is very fast (maximal power output in about 5 seconds) because few steps are involved and it doesn't need oxygen from blood. However, it is inefficient in terms of ATP yield, it creates lactic acid, which accumulates and eventually causes muscle fatigue.  It depletes muscle glycogen, which may take a day to recover after exhaustion. 

ATP made from aerobic breakdown of glycogen  is slow to get started because it requires 2 - 3 minutes for blood and oxygen supply to the muscle to increase and for many enzymes to start operating, but it yields almost 20x as much ATP per unit glycogen as anaerobic respiration. It also degrades lactic acid and reconverts it to glycogen, restoring optimum operating conditions and satisfying oxygen debt. 

Therefore, anaerobic respiration tends to supply ATP either in sudden, explosive exercise such as shot-putting, weightlifting, or sprinting, and long-term exercise is more aerobic. ATP supplied by anaerobic respiration accounts for different portions of different track events:

    1. 200 m dash (20 sec) is 90% anaerobic.
    2. Mile (4 minutes) is 50% anaerobic.
    3. 10 km run (29 minutes) is 10% anaerobic.
    4. Marathon (2.5 hours) is 2% anaerobic.

 


The above graphic is a synopsis of respiration.

Leaf tissue organization:  See an explanation of the following image here.
Inside these cells is where light energy from the ultimate energy source, the sun,
can be transfigured into chemical energy that's used by all living things.

Leaf Tissue Anatomy

Looking deeper into an individual plant cell:

Picture and Diagram of Chloroplast

 

The word "phosphorylation" is a fancy word for "adding a phosphorus to a molecule." 
You know this better as ADP + P --> ATP (for example).

Here's an easier version we did in class (below) of the above graphic.

Fermentation occurs when no O2 is present. 
It allows Glycolysis to continue,
yielding two ATPs for every one glucose molecule.